Ultra-restrictive red blood cell transfusion strategies in extensively burned patients

In recent years, due to the shortage of blood products, some extensive burn patients were forced to adopt an “ultra-restrictive” transfusion strategy, in which the hemoglobin levels of RBC transfusion thresholds were < 7 g/dl or even < 6 g/dl. This study investigated the prognostic impacts of ultra-restrictive RBC transfusion in extensive burn patients. This retrospective multicenter cohort study recruited extensive burns (total body surface area ≥ 50%) from three hospitals in Eastern China between 1 January 2016 and 30 June 2022. Patients were divided into an ultra-restrictive transfusion group and a restrictive transfusion group depending on whether they received timely RBC transfusion at a hemoglobin level < 7 g/dl. 1:1 ratio propensity score matching (PSM) was performed to balance selection bias. Modified Poisson regression and linear regression were conducted for sensitive analysis. Subsequently, according to whether they received timely RBC transfusion at a hemoglobin level < 6 g/dl, patients in the ultra-restrictive transfusion group were divided into < 6 g/dl group and 6–7 g/dl group to further compare the prognostic outcomes. 271 eligible patients with extensive burns were included, of whom 107 patients were in the ultra-restrictive transfusion group and 164 patients were in the restrictive transfusion group. The ultra-restrictive transfusion group had a significantly lower RBC transfusion volume than the restrictive transfusion group (11.5 [5.5, 21.5] vs 17.3 [9.0, 32.5] units, p = 0.004). There were no significant differences between the two groups in terms of in-hospital mortality, risk of infection, hospital length of stay, and wound healing time after PSM or multivariate adjustment (p > 0.05). Among the ultra-restrictive transfusion group, patients with RBC transfusion threshold < 6 g/dl had a significantly higher hospital mortality than 6–7 g/dl (53.1% vs 21.3%, p = 0.001). For extensive burn patients, no significant adverse effects of ultra-restrictive RBC transfusion were found in this study. When the blood supply is tight, it is acceptable to adopt an RBC transfusion threshold of < 7 g/dL but not < 6 g/dL.


Study participants
This retrospective study included patients at three hospitals.All three hospitals are tertiary care centers with specialized burn units and provide standardized treatment of extensive burn patients, including rapid airway establishment, early fluid resuscitation, scab excision, wound coverage, maintenance of organ function, infection control, and nutritional support therapy.Fluid resuscitation treatments were guided by the Third Military Medical University Fluid Resuscitation Formula, and adjusted according to individual conditions 18 .RBC transfusions were routinely used as treatment for anemia in which the transfusion thresholds were adjusted based on the current RBC reserves.Under conditions of adequate RBC reserves, a restrictive transfusion strategy was commonly employed, setting the transfusion threshold at 7-8 g/dl.In times of blood resource scarcity, RBC resources were coordinated by the hospitals' transfusion department, with the RBC transfusion threshold being lowered according to the actual degree of resource shortage.Intraoperative RBC transfusions were adjusted according to the intraoperative bleeding and hemodynamics of patients and were not limited by transfusion thresholds.
The inclusion criteria were as follows: (1) patients accepted inpatient treatment from 1 January 2016 to 30 June 2022 in the burn units of the three hospitals; (2) patients with TBSA burned ≥ 50%; (3) patients with complete electronic medical records.The exclusion criteria were as follows: (1) patients < 18 years of age; (2) pregnant patients; (3) patients who died within 72 h post-injury or within 24 h of admission; (4) patients admitted > 28 days post-injury; (5) patients discharged for personal reasons and patients whose prognoses could not be determined; (6) patients with recorded pre-existing anemia; (7) patients with a nadir hemoglobin level ≥ 8 g/dl; (8) patients with a nadir hemoglobin level ≥ 7 g/dl and without RBC transfusion during hospitalization (Fig. 1).
We followed the Strengthening the Reporting of Observational Studies in Epidemiology guidelines for conducting this retrospective cohort study.The research was approved by the Institutional Ethical Committee of the Second Affiliated Hospital of Zhejiang University College of Medicine (I20221060), the Institutional Ethical Committee of the First Affiliated Hospital of Nanchang University ((2023)CDYFYYLK(01-043)), and the Institutional Ethical Committee of the First Affiliated Hospital of Anhui Medical University (PJ 2023-10-12).All of the Institutional Ethical Committees waived the requirement for informed consent due to the observational, retrospective nature of this study.

Data collection
All information was collected using the same checklist from the electronic medical record systems of different hospitals.The following demographics and clinical characteristics were collected: gender, age, BMI (body mass index), TBSA burned, full thickness burned area, type of burn injury, inhalation injury, pulmonary edema, admission Multiple Organ Dysfunction Score (MODS), admission Acute physiology and chronic health evaluation II (APACHE II) score, revised Baux (rBaux) score, underlying disease, admission to the first operation, operation times and mechanical ventilation.Pulmonary edema was defined as the radiologically diagnosed accumulation of pulmonary interstitial fluid within 7 days after admission.The admission MODS and admission APACHE II scores were calculated based on parameters recorded during the first and second 24-h periods after admission 19,20 .rBaux scores were calculated as age + TBSA burned% + 17 (if inhalation injury) 21 .The hemoglobin values along with the time of each measurement, as well as the timing, volume, and adverse effects of each RBC transfusion during hospitalization were also collected.Nadir hemoglobin was defined as the lowest hemoglobin value measured during hospitalization.Preoperative hemoglobin was defined as the most recent hemoglobin value measured within 2 days before surgery, and postoperative hemoglobin was defined as the most recent hemoglobin value measured within 2 days after surgery.The hemoglobin difference between pre-and postoperative was defined as the value of preoperative hemoglobin minus postoperative hemoglobin.Hemoglobin before RBC transfusion was defined as the most recent hemoglobin value measured before each RBC transfusion event.
The prognostic variables were collected and included: in-hospital mortality, hospital length of stay (LOS), WHT, BSI, sepsis, wound infection, and catheter-related infection.WHT was defined as the time point at which wound closure by re-epithelisation reached 90%, or 7 days after the last excision and grafting treatment 7 .BSI, wound infection, and catheter-related infection were defined based on the isolation of bacteria or fungi from at least one culture 22 .Sepsis was defined as suspected infection and Sequential [Sepsis-related] Organ Failure Assessment(SOFA) score ≥ 2 23 .

Study design
'Ultra-restrictive transfusion' was defined as the absence of RBC transfusion for > 2 days in a patient with a hemoglobin level < 7 g/dl-below the level used in the restrictive transfusion strategy 7,13,14 .Patients who had experienced one or more 'ultra-restrictive transfusion' were assigned to the ultra-restrictive transfusion group, while other patients were assigned to the restrictive transfusion group.Subsequently, patients in ultra-restrictive transfusion group were stratified into < 6 g/dl group and 6-7 g/dl group based on whether they experienced the absence of RBC transfusion for > 2 days with a hemoglobin level < 6 g/dl.
The primary outcome measure of prognostic impact was in-hospital mortality.Secondary outcome measures included the incidences of BSI, sepsis, wound infection, and catheter-related infection, as well as hospital LOS and WHT determined in surviving patients (Fig. 1).

Statistical analysis
Multiple imputation was used to impute missing values, assuming randomly missing values, and using 10 imputed data sets.According to the Kolmogorov-Smirnov test, normally distributed continuous variables were expressed as medians ± standard deviation (SD); differences between groups were identified using the t-test.Non-normally distributed continuous variables were expressed as medians with interquartile ranges; differences between groups were identified using the Mann-Whitney U test.Categorical variables were expressed as numbers (percentages); comparisons were performed using the chi-squared test or Fisher's exact test, as appropriate.Violin plots were used to present the full distribution of the RBC transfusion volumes.To minimize the impacts of potential confounders and selection bias, propensity score matching (PSM) was used to compensate for differences in baseline patient characteristics between two group.A propensity score was calculated using logistic regression, and 1:1 patient matching was performed using the nearest-neighbor matching method without replacement.13 baseline characteristics, respectively gender, age, BMI, TBSA burned, type of burn injury, inhalation injury, pulmonary edema, admission MODS score, underlying disease, operation times, mechanical ventilation use, admission hemoglobin and hospital were matched and caliper radius equal to a standard deviation of 0.1 was set.Standardized differences were estimated before and after matching to evaluate balance, and a value of less than 0.2 indicated a balance between groups 24 .Kaplan-Meier and a log-rank test were used to compare survival between the two groups.Modified Poisson regression and linear regression were also conducted for sensitive  analysis.Baseline characteristics whose p-values between groups < 0.1 were included in the regressions for adjustment using the Enter method.Two-tailed p values < 0.05 were considered indicative of statistical significance.All statistical analyses were performed using SPSS software (version 27) and R software (4.2.2).

Demographics and clinical characteristics
Among the 494 patients admitted to the three hospitals between 1 January 2016 and 30 June 2022, 223 were excluded: 7 patients were < 18 years of age, 6 patients died within 72 h post-injury or within 24 h of admission, 3 patients were admitted > 28 days post-injury, 10 patients unable to determine prognosis, and 197 patients with a nadir hemoglobin level ≥ 8 g/dl or a nadir hemoglobin level ≥ 7 g/dl without RBC transfusion during hospitalization.
The general demographic data and clinical characteristics of the 271 remaining eligible patients are shown in Table 1.Among them, 180 (66.4%) were men, 91 (33.6%) were female, the mean of age was 45.1 ± 13.9 years.The median TBSA burned area was 75.0%(64.3%, 90.0%) and the mean of rBaux score was 147.6 ± 20.6.107 patients who did not receive an RBC transfusion for > 2 days despite a hemoglobin level < 7 g/dl were assigned to the ultra-restrictive transfusion group; the remaining 164 patients were assigned to the restrictive transfusion group.Compared with the restrictive transfusion group, the proportion of males, the admission MODS scores, and the rate of pulmonary edema were all higher in the ultra-restrictive transfusion group (p < 0.05).

Hemoglobin levels and transfusions of ultra-restrictive transfusion group and restrictive transfusion group
The nadir hemoglobin level was significantly lower in the ultra-restrictive group than in the restrictive transfusion group (5.8 [5.3, 6.2] vs 7.1 [6.3, 7.5] g/dl, p < 0.001).Patients in the ultra-restrictive transfusion group had a longer period in which their hemoglobin was < 7 g/dl, while patients in the restrictive transfusion group hardly had such time (6.0 [2.0, 12.0] days vs 0.0 [0.0, 1.0] days).Notably, only 8 patients underwent the 'ultra-restrictive transfusion' in the first 2 days postoperative.The nadir preoperative hemoglobin was 6.9 (6.5, 7.6) g/dl in the ultra-restrictive transfusion group and 8.7 (8.0, 9.6) g/dl in the restrictive transfusion group, while the nadir postoperative hemoglobin was 7.1 (6.3, 7.9) g/dl in the ultra-restrictive transfusion group and 7.8 (7.0, 8.4) g/ dl in the restrictive transfusion group.The mean hemoglobin difference between pre-and postoperative was 0.6 (− 0.1, 1.3) g/dl in the ultra-restrictive transfusion group and 0.9 (0.1, 1.8) g/dl in the restrictive transfusion group with no significant differences between the two groups (p = 0.105).
Results of the modified Poisson regressions and linear regressions without and with adjustment are shown in Table S2.After adjustment for covariates including gender, BMI, TBSA burned, pulmonary edema, admission MODS score, and the hospital, there were no significant associations between ultra-restrictive transfusion and all prognostic outcome indicators (p > 0.05).

Effect of RBC transfusion threshold < 6 g/dl on prognosis
Among the ultra-restrictive transfusion group, there were 32 patients did not receive an RBC transfusion for > 2 days despite a hemoglobin level < 6 g/dl.The Baseline characteristics between < 6 g/dl group and 6-7 g/ dl group had no significant differences (p > 0.05).(Table S3) < 6 g/dl group had a significantly higher hospital mortality than 6-7 g/dl group (53.1% vs 21.3%, p = 0.001).The Kaplan-Meier curves also showed significant survival differences between the two groups (p = 0.002).(Fig. 4) There were no significant differences in other prognostic outcome indicators between < 6 g/dl group and 6-7 g/dl group (p > 0.05) (Table 3).

Discussion
This retrospective multicenter study investigated the prognostic impacts of ultra-restrictive RBC transfusion in extensive burn patients (TBSA > 50%).According to the comparison between the ultra-restrictive and restrictive RBC transfusion group, we found that an RBC transfusion threshold < 7 g/dl had no significant adverse effects for extensive burn patients while an RBC transfusion threshold < 6 g/dl may increase the mortality risk.Although the potential risk of setting 6-7 g/dl as the transfusion threshold cannot be ruled out, the results will provide a valuable reference for the rational allocation of limited RBC products in extensive burn patients.TBSA is one of the most important indicators of the severity of burns.Patients with extensive burns often exhibit strong systemic reactions, severe anemia, and high mortality rates after injury.Their treatment often involves massive transfusions of blood products.A large Chinese burn center reported that from 2003 to 2009, the mortality of patients with burns to ≤ 50% of the TBSA was only 0.39%, but 32.19% among those with burns to > 50% of the TBSA 25 .Based on the severity criteria for burns in our country, patients with TBSA more than    www.nature.com/scientificreports/50% are specifically classified as critically severe and are usually treated in the burn intensive care unit after the injury 25,26 .Given that this study used mortality and other prognostic outcomes as measures, to prevent less severe patients from masking the impact of transfusion strategies on the prognosis of critically burned patients, this study only included patients with TBSA ≥ 50%.Compared with previous studies of patients with TBSA ≥ 20%, the extensive burn patients in our study had obviously higher mortality and infection rates, longer in-hospital LOSs and WHTs 7,12 .
The RBC transfusion protocol should be designed to ensure optimal clinical outcomes while also minimizing unnecessary RBC transfusions, which may increase the risk of adverse transfusion reactions, as well as waste blood resources and increase costs 6,14 .According to the clinical guidelines from the American Association of Blood Banks in 2016, a total of 31 RCT studies (involving 12,587 participants) compared restrictive transfusion thresholds with liberal transfusion thresholds.Their cross-trial analysis indicated that restrictive RBC transfusion thresholds were not associated with higher rates of adverse clinical outcomes 27 .Subsequently, the TRIBE trial included 347 patients with > 20% TBSA burns to compare these two transfusion strategies (restrictive transfusion threshold set as 7 g/dl; liberal transfusion threshold set as 10 g/dl) in burn patients.The results showed that there were no statistically significant differences in all of the outcome measures, while the restrictive transfusion group received significantly fewer RBC transfusions (3411 vs. 5636 total, median 8 vs 16 units/patient).Based on their estimations, the use of a restrictive blood transfusion strategy in critically ill burn patients in the United States could potentially lead to annual cost savings of approximately $31,543,220 to $47,314,680 7 .Another RCT study included 80 patients with TBSA > 20% and set the restrictive transfusion threshold as 8 g/dl, and the liberal transfusion threshold as 10 g/dl.The mean number of RBC unit transfusions per patient in the restrictive group was significantly lower than in the liberal group (3.28 units vs 5.9 units).They also found no significant Figure 4. Kaplan-Meier survival curves for patients in 6-7 g/dl group and < 6 g/dl group.The dark blue line represents the Kaplan-Meier survival curve in < 6 g/dl group, and the red line represents the Kaplan-Meier survival curve in 6-7 g/dl group.The light blue and light red areas represented the 95% confidence intervals.Log-rank p = 0.02.Table 3. Outcome measures of 6-7 g/dl group and < 6 g/dl group.Data are shown as the median (25th percentile, 75th percentile) or the number of patients (%), as appropriate.BSI blood stream infection, LOS length of stay, WHT wound healing time.difference in mortality rate or other outcome measures between groups 12 .However, in recent years, the shortage of blood resources has led clinical transfusion thresholds being set lower than those established in the aforementioned studies.As a result, such that some of our patients did not receive RBC transfusions despite a hemoglobin level < 7 g/dl 28 .Especially during the years 2019-2022 with the COVID-19 pandemic, decreased blood donations and increased demand for blood products led to an increase in the number of these patients 28 .
In previous studies, the hemoglobin threshold established in a 'restrictive transfusion strategy' was 7.0-8.0g/ dl; therefore, the present study regarded an RBC transfusion threshold < 7 g/dl as 'ultra-restrictive transfusion' 7,13,14 .Considering the delay between hemoglobin measurement and RBC transfusion, this group consisted of patients who did not receive an RBC transfusion for > 2 days after a hemoglobin level < 7 g/dl was recorded.As expected, patients in the ultra-restrictive transfusion group had significantly lower RBC transfusion thresholds and received significantly smaller RBC volumes.However, the prognostic outcomes did not significantly differ between the two groups with respect to in-hospital mortality, survival time, hospital LOS, and WHT.Infection is another important factor to consider when evaluating RBC transfusion strategies.It is generally believed that transfusion increases the risk of infection through exposure to potentially contaminated blood products and immunomodulatory effects 11,29 .Nevertheless, in the majority of previous RCT studies comparing restrictive transfusion with liberal transfusion, there was no correlation between infection and RBC transfusion strategy in patients 7,30 .Notably, the crude incidences of BSI, wound infection, and catheter-related infection were higher in the ultra-restrictive group.Considering that the infection rates were associated with the culture frequency, which varies widely among different hospitals, we included the hospital in the adjustment together with other baseline characteristics.After PSM or adjusted modified Poisson regressions, there were no significant differences in all types of infection rates between the two groups which was similar to the previous studies.Additionally, among the 271 patients, there were no transfusion-induced adverse events, although the potential risk of transfusion could not be definitively ruled out.
Although our results showed no significant adverse effects of ultra-restrictive RBC transfusion, there is no doubt that the RBC transfusion threshold cannot be lowered indefinitely.When the severity of anemia surpasses the patient's tolerance, it can result in severe consequences such as tissue hypoxia due to insufficient oxygen transport delivery of blood 6,13 .The hypermetabolism, cardiac dysfunction, and severe infections experienced by extensive burn patients may result in a lower tolerance to anemia compared to normal individuals 31 .Thus, we subsequently investigated the prognostic impact of RBC transfusion threshold < 6 g/dl in the ultra-restrictive transfusion group.The results revealed a significant increase in mortality among patients who experienced the absence of RBC transfusion with a hemoglobin level < 6 g/dl.This suggests that lowering the transfusion threshold below 6 g/dl may not be advisable.
Overall, our findings suggest that implementing a transfusion threshold of 6-7 g/dl may alleviate the strain on RBC resources and reduce associated costs in patients with extensive burns, without impacting prognostic outcomes, in comparison to a transfusion threshold of 7-8 g/dl.Although COVID-19 was successfully curtailed, blood scarcity is still a global and long-established problem that involves multiple factors including donor recruitment, blood collection, blood testing, blood processing, blood distribution, blood transfusion management, and so on 17,32 .A modeling study showed that in 2017, the global unmet demand for blood products was equivalent to 1849 units per 100,000 population 33 .The treatment of extensive burn patients requires massive RBC volumes.In our study, although most of the patients had adopted RBC transfusion with a low hemoglobin threshold, the mean RBC transfusion volume was 16 units, much was much higher than the requirement of intensive care unit admission patients and burn patients with TBSA > 20% (ranged from 2 to 4 units) 2,34,35 .When the number of extensive burn patients is relatively large, especially after mass casualty burn incidents (e.g., fire and war), the demand for RBC for the treatment of extensive burns will inevitably put pressure on the allocation of blood resources 36 .At this time, based on our research findings, adopting an RBC transfusion threshold of 6-7 g/dl may help optimize the allocation of blood resources and improve the treatment of more patients.In addition to extensive burns, ultra-restrictive RBC transfusions were also forced to be used in various types of patients.(e.g., patients with tumors, pregnancy-related complications, trauma, gastrointestinal bleeding, sepsis) 17,28 .Considering most clinical studies comparing restrictive and liberal transfusion strategies have reached similar conclusions in different populations, it is believed that our research findings will provide valuable references for subsequent studies on lowering the RBC transfusion threshold in other populations 6,9 .
It is worth noting that the appropriateness of ultra-restrictive RBC transfusion in the perioperative period warrants consideration.Intraoperative blood loss is a major factor contributing to anemia in patients with extensive burns.According to our previous analysis, the median number of surgeries for patients with extensive burns in the three hospitals was 3, with a median intraoperative blood loss of 2541 ml per procedure.Intraoperative blood loss was identified as an independent factor contributing to an increase in RBC transfusion volume for these patients 37 .In the present study, patients demonstrated significantly higher levels of hemoglobin preoperatively and postoperatively compared to their nadir hemoglobin levels.The postoperative hemoglobin levels were basically controlled above 7 g/dl, with only 8 patients subjected to ultra-restrictive RBC transfusion within 2 days after surgery.These results suggested that the perioperative RBC transfusions were not significantly impacted by blood resource constraints, which may be related to the strict preoperative evaluation of patients and the fact that intraoperative RBC transfusion is not limited by hemoglobin threshold.In the previous RCT studies about transfusion strategies in burn patients, RBC transfusions during surgical procedures were similarly not restricted by the defined hemoglobin threshold due to safety considerations 7,12 .Due to the rapid blood loss during surgery leading to a rapid decrease in blood volume, and the potential decrease in patient tolerance to anemia due to surgical stress, restrictions on transfusion thresholds must be used cautiously during intraoperative and perioperative periods.Furthermore, elderly individuals may have decreased physiological function and diminished organ reserves, making them less capable of tolerating low hemoglobin levels compared to young adults 38 .It is important to note that the proportion of elderly patients in our study was relatively small, and therefore, the generalizability of results to elderly patients also warrants consideration.
This study has some limitations.First, due to the retrospective nature of the study, our grouping method could only be based on whether patients received RBC transfusions at a certain hemoglobin level, rather than their target RBC transfusion thresholds.This grouping method may have reproduced the selection bias of clinicians, who often prioritize the allocation of blood resources to patients in a worse state of illness 39 .Despite being adjusted for covariates related to the different admission characteristics and treatment processes of the two groups, differences in their dynamic conditions could not be completely eliminated.Additionally, the frequency of hemoglobin measurements may impact the grouping, as patients in the ultra-restrictive transfusion group may be misclassified due to inadequate hemoglobin measurement frequency.Second, due to the small number of patients with a transfusion threshold < 6 g/dl, more in-depth statistical analyses (e.g., the adjustment of baseline characteristics and the analysis of mortality risk factor) could not be performed.The specific impact of transfusion threshold < 6 g/dl or lower on extensive burn patients cannot be identified.Third, again due to the limited sample size, the subgroup analysis was not carried out in the present study, thus failing to consider the individualization of transfusion thresholds 31 .Previous studies showed greater anemia tolerance by women than men and by younger adults than older adults 38,40 .Whether ultra-restrictive transfusion has adverse effects in these populations remains uncertain.Fourth, the transfusion strategies in this study were based solely on the hemoglobin level.Other factors including the degree of blood concentration, the causes and progression rate of anemia, as well as the drug usage including iron supplements and erythropoietin should also be considered when choosing the transfusion strategy 31,41 .Fifth, patients were not followed up after discharge from the hospital; thus, the long-term complications of ultra-restrictive transfusion still cannot be ruled out 42,43 .Future large-sample and multicenter studies are warranted to address these limitations and determine the optimal RBC transfusion protocol.Patients should be classified according to various clinical characteristics that may affect anemia tolerance (e.g., gender, age, TBSA), and the lowest hemoglobin threshold that each subgroup of patients can tolerate without affecting prognosis should be determined.In addition to focusing on outcome indicators during hospitalization, long-term hemoglobin levels and cardiopulmonary function after discharge should also be taken into consideration.

Conclusions
Our results showed that, for extensive burns, ultra-restrictive transfusion with an RBC transfusion threshold < 7 g/dl had no significant effect on hospital mortality, hospital LOS, WHT, and risk of infection.However, an RBC transfusion threshold < 6 g/dl may increase the mortality risk.When the blood supply is tight, it is acceptable to adopt an RBC transfusion threshold of < 7 g/dl but not < 6 g/dl.In addition, prospective cohort multicenter studies to better define the use of restrictive and ultra-restrictive RBC transfusion strategies are recommended.

Figure 1 .
Figure 1.Flowchart of participants selected and data analysis.TBSA total body surface area, RBC red blood cell.

Figure 2 .
Figure 2. Violin plots of RBC transfusion volumes in two groups.The distributions of RBC transfusion volumes are represented by violin plots.The thicker line in the middle of the box is the median, the upper and lower boundaries of the box represent the third and the first quartile, upper and lower whiskers represent the quartile plus 1.5 interquartile (IQR) and first quartile minus IQR, and each gray dot represents the RBC transfusion volume of a single patient.The total RBC transfusion volume, operating room RBC transfusion volume non-operating room RBC transfusion volume in ultra-restrictive transfusion group were significantly lower than restrictive transfusion group.RBC red blood cell.

Figure 3 .
Figure 3. Kaplan-Meier survival curves for patients in ultra-restrictive transfusion group and restrictive transfusion group before and after PSM.The dark blue lines represent the Kaplan-Meier survival curves in ultra-restrictive transfusion group, and the red lines represent the Kaplan-Meier survival curves in restrictive transfusion group.The light blue and light red areas represented the 95% confidence intervals.Before PSM Logrank p = 0.157; After PSM.Log-rank p = 0.810.PSM propensity score matching.

Table 1 .
Baseline characteristics, hemoglobin levels and transfusions of ultra-restrictive transfusion group and restrictive transfusion group.Data are shown as the median (25th percentile, 75th percentile), mean ± standard deviation or the number of patients (%), as appropriate.BMI body mass index, TBSA total body surface area, MODS multiple organ dysfunction score, APACHE II Acute physiology and chronic health evaluation II, rBaux revised Baux, RBC red blood cell.

Table 2 .
Outcome measures of ultra-restrictive transfusion group and restrictive transfusion.Data are shown as the median (25th percentile, 75th percentile) or the number of patients (%), as appropriate.PSM propensity score matching, BSI blood stream infection, LOS length of stay, WHT wound healing time.